Disease. Gummy stem blight. Causes leaf spot, stem canker, black leaf and fruit rot; common name refers to the characteristic gummy exudate. Didymella bryoniae (Auersw.) Rehm [anamorph Phoma cucurbitacearum (Fr.) Sacc.] is the causative agent of gummy stem blight (GSB), a disease affecting. Gummy stem blight of cucurbits produces a variety of symptoms which are referred to as leaf spot, stem canker, vine wilt and black fruit rot. Lesions on leaves.

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There is considerable variation in fruit susceptibility to black rot within C. American Phytopathological Society, St. In addition to the six commonly cultivated cucurbit species listed above, foliar or crown lesions and black rot have been reported on bitter melon also known as balsam pear Momordica charantiabottle gourd Lagenaria sicerariasmooth loofah Luffa cylindricaand chayote also known as vegetable pear Sechium edule 2,14,22, GSB produces lesions on the stems and leaves, and can also be spread by seeds.

First report of gummy stem blight, caused by Didymella bryoniaeon watermelon transplants in California. The designed primer sequences for D. The samples were prepared as described above.

BoxArusha, Tanzania Go to article: This page was last modified To obtain a pure culture from a single conidium, a 3-mm-square piece is cut from an area of the colony with pycnidia, taking care to avoid other microorganisms if an isolation plate is used as the source.

Diagnostic guide for gummy stem blight and black rot on cucurbits. Color and shape of leaf spots vary somewhat with host, but the following characteristics are common. No use, distribution or reproduction is permitted which does not comply with these terms.


Template DNA from D. Infection also occurs on seedlings. Sugar Baby and Charleston Grey and pumpkin cv. Based on work by John and Michasia Harris and others. In the older literature, Mycosphaerella melonis Pass.

The LAMP assay established in this bryoniaw can be used for numerous applications, such as the potential field use for efficient GSB diagnosis and management, seed quarantine, and evaluation of GSB resistance in breeding procedures.

Flowers and fruits of both hosts developed black rot spots and aborted.

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Descriptions of Pathogenic Fungi and Bacteria. The fungus was reisolated from infected leaves and stems of both hosts. Author information Article notes Copyright and License information Disclaimer.

The reaction mixtures in the microcentrifuge tubes were visually inspected by the naked eye to determine the color change. Optimal reaction time of LAMP.

In the current study, a credible, sensitive, and broad-spectrum diagnostic method for the detection of D. Fungal plant pathogens and diseases Food plant pathogens and diseases Pleosporales. Muskmelon seedlings were inoculated with the two genotypes of D.

Didymella bryoniae

Benincasa hispida wax gourd. Each product was confirmed by 2.

This pathogen commonly affects the foliage and stems of plants from the family Cucurbitaceae the family of gords and melonswhich includes cantaloupe, cucumber, muskmelon and watermelon plants [1,3,8]. The center of spots often is a lighter shade of brown than the surrounding portion Figs.

Assessment was based on A fluorescence metal indicator calcein visualization of color change, B the turbidity analysis of the LAMP products or C agarose gel electrophoresis analysis of the LAMP products. Retrieved from ” https: Thus, there is a need to develop didmella straightforward, sensitive, rapid, and cost-effective method for the early diagnosis and in situ testing of D.


Early symptoms appear as yellowing or chlorosis of the leaf margins btyoniae by light to dark brown necrotic spots. Leaf spots of gummy stem blight are triangular when they occur at the leaf margin Hubbard squash, Cucurbita maxima. Compendium of Cucurbit Diseases, T. A photoperiod ddymella 16 h of light is required to induce rapid production of fruiting bodies 10, Lesions usually form at the joints on the stem, dieymella elongated, cracked, and exude amber colored sap. Hence, this study represents a successful attempt to develop a LAMP-based detection method of infection in early cucurbit crops.

Walker, Phyllosticta citrullina Chester, Sphaerella bryoniae Auersw. Gummy stem blight GSB of cucurbits. This improved the reaction time and efficiency.

Didymella bryoniae – Bugwoodwiki

Electrochemical behavior of calcein and the interaction between calcein and DNA. Didymella bryoniae survives on deceased vines, crop debris and on seeds in between seasons and D.

The results demonstrated that this method is specific and efficient. Genomic DNA of D. Notes on three new or noteworthy diseases of plants. Didymslla detection of Didymella bryoniae in cucurbit seeds using a loop-mediated isothermal amplification assay.