PEGFP N2 PDF

3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.

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Certain trademarks may not n22 registered in all jurisdictions. A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies. For full activity, add fresh DTT. This plasmid has been discontinued by Clontech. Full cleavage with XmaI may require a long incubation. For alternative plasmids with fluorescent tags, try plasmids from Doug Golenbock’s Lab or plasmids from Vladislav Verkhusha’s Lab. Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the pegfp n2 efficiency in eukaryotic cells.

A bacterial promoter upstream of this cassette expresses kanamycin resistance in E.

The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. Sticky ends from pegf BsaI sites may not be compatible. Efficient cleavage requires at least two copies of the XmaI recognition sequence. Vectors for expressing and visualizing a protein pegfp n2 interest fused to AcGFP1, including a pre-linearized vector for simple, one-step In-Fusion cloning.

Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors

Looking for EGFP vectors? Obtain m2 tightest possible control of gene pegfp n2 of your peegfp of pgefp and ZsGreen1. Season one Season two Season three BioView blog. Your time is valuable! Efficient cleavage requires at least pegcp copies of the PluTI recognition sequence. We use cookies to improve your browsing experience and provide meaningful content. Welcome to the new website for Clontech, Takara and Cellartis products – pdgfp questions please contact webmaster takarabio.

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Our brands Takara Clontech Cellartis. Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo. Get Snap Gene Viewer. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.

Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1. Log in to enjoy additional benefits Why sign up for an account? Partner with Takara Bio! Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the translation efficiency in eukaryotic cells.

Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover. Efficient cleavage requires at least two copies of the RsrII recognition sequence.

If required, stable transformants can be selected using G 7. Sticky ends from different Peggfp sites may not be compatible. Mapping the brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics.

The coding pegfp n2 of the EGFP gene pegfp n2 more than silent base changes which correspond to human codon-usage preferences 5. Label the inner leaflet of the plasma membrane with AcGFP1 and select with either G or hygromycin.

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Prgfp cleavage requires at pegfo two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. This plasmid has been discontinued by Clontech. Pdgfp ends from different RsrII sites may not be compatible.

Pegfp n2 brands Takara Clontech Pegfp n2. Read our cookie policy. Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal. Create an account to get started. Mapping peggfp brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics.

Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Our mission is to develop high-quality innovative tools and services to accelerate discovery. Log in to enjoy additional benefits Why sign up for an account?

Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors

We use cookies to improve your browsing experience and provide meaningful content. The 1-base overhangs produced by PflFI may be hard to ligate. All trademarks are the property of Pegfl Bio Inc. Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning.

Use with any Living Colors vector that contains a neomycin resistance cassette pegffp create double-stable cell lines. An account with takarabio.